Hepatitis B surface antigen (HBsAg) concentrations were measured using a piezoelectric microcantilever sensor (PEMS) developed\r\nby the authors. The developed PEMS is label-free and detects the sensing signal electrically. It was designed to measure the mass of\r\nbiomolecules attached to it using an accurate mass-microbalancing technique; its probe area is confined to the end of the cantilever,\r\nand its equivalent spring constant is relatively high to minimize the effect of changes in the surface stress when the biomolecules\r\nare attached to it. The ââ?¬Å?dip- and-dryââ?¬Â technique was used to enable the probe area of the sensor to react with reagents in controlled\r\nenvironmental conditions. HBsAg was detected by an immunoreaction whereas the reaction time, antibody density, and its area\r\non the probe were kept at a constant level. The mass of the detected HBsAg was measured in the range of 0.1ââ?¬â??100 ng/mL.
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